9873

A Selection and Analysis Tool for Fluorescence Recovery after Photobleaching (FRAP) Region of Interest (ROI)

Fluorescence recovery after photobleaching (FRAP) can be used to quantify the mobility of molecules in live cells. In a typical FRAP experiment, a portion of a cell expressing a fluorescently tagged molecule of interest is photobleached and the recovery of the photobleached region is monitored as a function of time. Briefly, faster recoveries correspond to more mobile molecules. In this Demonstration a movie of a FRAP experiment is analyzed. You can scroll through the movie in the upper display and choose the size and position of a circular region of interest (ROI). The normalized intensity of the fluorescence in the ROI is displayed as a function of image number in a plot below. A vertical red line designates the current image number of the movie in the upper display.

THINGS TO TRY

SNAPSHOTS

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DETAILS

For details on FRAP, see the Wikipedia article "Fluorescence Recovery after Photobleaching".
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