Fluorescence Analysis Tool for Bio-Imaging

Requires a Wolfram Notebook System

Interact on desktop, mobile and cloud with the free Wolfram CDF Player or other Wolfram Language products.

Requires a Wolfram Notebook System

Edit on desktop, mobile and cloud with any Wolfram Language product.

This Demonstration is intended to be used for bio- and medical-imaging analysis. The program automatically creates thresholds for the given image, determining the segments of bright fluorescence. Click a segment and the computer will calculate the size (in pixels), the total fluorescence, and the fluorescence per pixel of the selected segment. You can vary the thresholding parameters, "foreground threshold" and "size threshold". You can switch between the two images that can be loaded using the "image number" buttons. To see all the calculated data, click the "data list" button.

[more]

The program will store some intermediate calculation results so that manipulation runs faster. After the first time, it simply reloads and shows the data. If threshold parameters are changed, calculation will be restarted.

[less]

Contributed by: Masato Ohgishi (August 2011)
Based on a program by: Shadi Ashnai
Open content licensed under CC BY-NC-SA


Snapshots


Details

detailSectionParagraph


Feedback (field required)
Email (field required) Name
Occupation Organization
Note: Your message & contact information may be shared with the author of any specific Demonstration for which you give feedback.
Send