9853

Saturation Binding of Ligands to Proteins

Basic saturation binding experiments to determine dissociation constants ) assume that the concentration of free ligands is about the same (greater than 90%) as the concentration of ligands added. If not, the results could be invalid due to ligand depletion. A general rule of thumb is to use a starting concentration of protein well below (~10 times) the estimated , and ligand concentrations that bracket the by at least 10 times on either side. Many published experiments ignore this rule. This graphical aid simplifies experimental design by readily showing safe macromolecule/ligand concentrations in black and dangerous conditions in red. , , and all have the same units of concentration (nM in this Demonstration).

SNAPSHOTS

  • [Snapshot]
  • [Snapshot]
  • [Snapshot]

DETAILS

The equation used is
References
[1] K. B. Hall and J. K. Kranz, "Nitrocellulose Filter Binding for Determination of Dissociation Constants," Methods in Molecular Biology, 118, 1999 pp. 105–114.
[2] H. Motulsky, "GraphPad Prism Version 4.0 Statistics Guide," La Jolla, CA: GraphPad Software, Inc., 2005.
    • Share:

Embed Interactive Demonstration New!

Just copy and paste this snippet of JavaScript code into your website or blog to put the live Demonstration on your site. More details »

Files require Wolfram CDF Player or Mathematica.









 
RELATED RESOURCES
Mathematica »
The #1 tool for creating Demonstrations
and anything technical.
Wolfram|Alpha »
Explore anything with the first
computational knowledge engine.
MathWorld »
The web's most extensive
mathematics resource.
Course Assistant Apps »
An app for every course—
right in the palm of your hand.
Wolfram Blog »
Read our views on math,
science, and technology.
Computable Document Format »
The format that makes Demonstrations
(and any information) easy to share and
interact with.
STEM Initiative »
Programs & resources for
educators, schools & students.
Computerbasedmath.org »
Join the initiative for modernizing
math education.
Step-by-step Solutions »
Walk through homework problems one step at a time, with hints to help along the way.
Wolfram Problem Generator »
Unlimited random practice problems and answers with built-in Step-by-step solutions. Practice online or make a printable study sheet.
Wolfram Language »
Knowledge-based programming for everyone.
Powered by Wolfram Mathematica © 2014 Wolfram Demonstrations Project & Contributors  |  Terms of Use  |  Privacy Policy  |  RSS Give us your feedback
Note: To run this Demonstration you need Mathematica 7+ or the free Mathematica Player 7EX
Download or upgrade to Mathematica Player 7EX
I already have Mathematica Player or Mathematica 7+