A Selection and Analysis Tool for Fluorescence Recovery after Photobleaching (FRAP) Region of Interest (ROI)

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Fluorescence recovery after photobleaching (FRAP) can be used to quantify the mobility of molecules in live cells. In a typical FRAP experiment, a portion of a cell expressing a fluorescently tagged molecule of interest is photobleached and the recovery of the photobleached region is monitored as a function of time. Briefly, faster recoveries correspond to more mobile molecules. In this Demonstration a movie of a FRAP experiment is analyzed. You can scroll through the movie in the upper display and choose the size and position of a circular region of interest (ROI). The normalized intensity of the fluorescence in the ROI is displayed as a function of image number in a plot below. A vertical red line designates the current image number of the movie in the upper display.

Contributed by: Timothy J. Stasevich (April 2011)
Open content licensed under CC BY-NC-SA



For details on FRAP, see the Wikipedia article "Fluorescence Recovery after Photobleaching".

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