Tetl-Box Synthetic Bacteria Growth Kinetics

This Demonstration shows plots of the bacterial growth of Escherichia coli cells that contain synthetic plasmids. This is part of the Tetl-Box project [1] for producing laboratory reagents.
Use the sliders to select the parameters for synthetic organism growth: the source of energy and carbon ("substrate concentration"), selective pressure ("antibiotic concentration") and biocontainment level ("lactose concentration" and "completion time" when lactose will be added).


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Tetl-Box is the Ollin SynBio IPN team project for the synthesis of laboratory reagents using bacteria containing synthetic plasmids [1]. This was a project entered in the iGEM Design League competition.
The growth of bacteria containing a synthetic plasmid can be manipulated by varying the concentration of its carbon and energy source (glucose), the concentration of the antibiotic that maintains the selective pressure (because some bacteria will lose the plasmid and compete for resources), the lactose concentration that will kill the synthetic bacteria (the plasmid contains a caspase-based biocontainment system and the LacI regulator) and the time lactose will be added to end the experiment. This simulation is based on the mathematical models discussed in [2–4].
[1] Ollin SynBio IPN. "Tetl–Box." (Oct 8, 2021) app.jogl.io/project/741/TetlBox.
[2] P. M. Doran, Bioprocess Engineering Principles, 2nd ed., Boston: Academic Press, 1995.
[3] K. Han and O. Levenspiel, "Extended Monod Kinetics for Substrate, Product, and Cell Inhibition," Biotechnology and Bioengineering, 32(4), 1988 pp. 430–447. doi:10.1002/bit.260320404.
[4] N. G. McDuffie, Bioreactor Design Fundamentals, Boston: Butterworth-Heinemann, 1991.
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