Two-State Protein Melting Curve (N, P, T Ensemble)

Initializing live version
Download to Desktop

Requires a Wolfram Notebook System

Interact on desktop, mobile and cloud with the free Wolfram Player or other Wolfram Language products.

This Demonstration illustrates the fraction () of a two‐state protein (or other biomolecule) that has melted at temperature . The two states of the protein are denoted (native or folded) and (denatured or melted) and we consider the dynamic equilibrium . The ratio of the two states' equilibrium concentrations is , where ) is the standard state Gibbs free energy difference and is the universal gas constant (8.314 J/mol-K). Melting occurs when (i.e., ). . The parameter (standard state enthalpy difference) controls the steepness of the transition, but does not affect the location of the point where . The temperature () at which is and .

Contributed by: David L. Pincus (March 2011)
Open content licensed under CC BY-NC-SA



Feedback (field required)
Email (field required) Name
Occupation Organization
Note: Your message & contact information may be shared with the author of any specific Demonstration for which you give feedback.