The characteristics of continuous operation are as follows:
Snapshot 1: steady state; after an initial startup period, there is no variation of concentrations with time
Snapshot 2: constant reaction rates, where
is the biomass reaction rate,
is the reaction rate of the consumed reactant and
is the product reaction rate
Snapshot 3: Washout of the organisms (cells) will occur when the dilution rate
is greater than the specific growth rate
. This corresponds to the complete removal of cells by flow out of the tank. In many references, including , the variables
are plotted versus the dilution rate
, which characterizes the steady state in a chemostat. See Related Links.
Some suggestions for the user:
interactively to note effect on washout.
Change the feed substrate concentration
to alter the steady state.
Investigate the influence of maintenance requirements on the steady-state biomass concentration.
Operate initially as a batch reactor with
, and switch to chemostat operation with
is the maximum specific growth rate, and in this case was set up to
, the biomass-substrate yield, making use of the yield graph.
 I. J. Dunn, E. Heinzle, J. Ingham and J. E. Přenosil, Biological Reaction Engineering: Dynamic Modelling Fundamentals with Simulation Examples, Second Edition
, Weinheim, Germany: Wiley-VCH, 2003.